"Organ-on-chip strategy to construct high-fidelity in vitro models recapitulating native microenvironment conditions : example of the vascularized liver sinusoid"
Biomimetism is now crucial in biomedical research. Thanks to recent progress in microtechnology and materials science and engineering, it is indeed possible to reproduce natural cellular microenvironments in vitro in a controllable manner. New tools and cell culture platforms are now being developed not only to understand complex biological processes at different time and spatial scales but also to greatly improve the fidelity of all the physical cues that are responsible for the biological mechanisms under study, showing that cell signalling is also physical and not only chemical.
In order to control all these mechanical, geometrical and dynamic properties impacting biological processes in physiopathology, technology now offers to improve the quality and fidelity of cell culture platforms, enabling a recapitulation of processes happening all the time in vivo and at all scales. It is now possible to access, study and engineer the physiological microenvironment in culture, from the microscopic structural architecture of the extracellular matrix (constituting the cell-supporting adhesive layers) up to the macroscale flows and deformations impacting cells collectively at the tissue-scale to the organ size.
In this seminar, we will present some progress made towards the development of a liver-sinusoid-on-chip platform recapitulating the mechanical stimuli known to influence sinusoid cells to identify the correct conditions that will enable the maintenance of hepatic cells functional phenotype and then study liver pathologies in vitro.
Séminaire retransmis en visioconférence : https://u-bordeaux-fr.zoom.us/j/86132527651