Séminaire IBGC "Exploring the non-coding genome through long-read sequencing"

"Exploring the non-coding genome through long-read sequencing"

Long-read sequencing is opening new frontiers in transcriptome analyses by enabling the direct observation of full-length RNA molecules, thereby overcoming some limitations of short-read assembly. In this talk, I will present recent work from our group that combines long-read technologies with novel computational tools to uncover hidden layers of transcriptome complexity. I will introduce ANNEXA, a method we developed for context-specific annotation of long non-coding RNAs (lncRNAs) and will highlight our benchmark results on circular RNA (circRNAs) discovery with long-read RNASeq data. These approaches highlight how long-read technologies can deepen our understanding of the non-coding transcriptome, with accurate annotation as a necessary step toward functional exploration.